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Deduced functions of proteins encoded by the MM 4550 biosynthetic gene cluster

Journal: Chembiochem : a European journal of chemical biology

Article Title: Identification and characterization of the carbapenem MM 4550 and its gene cluster in Streptomyces argenteolus ATCC 11009

doi: 10.1002/cbic.201300319

Figure Lengend Snippet: Deduced functions of proteins encoded by the MM 4550 biosynthetic gene cluster

Article Snippet: Protein Amino acids Proposed function Sequence similarity (identity/positive) Identity/ positive to ATCC 33331(%) Orf1 238 Unknown No similar sequences --/-- a Orf2 475 arabinofuranosidase SMCF_639, Streptomyces coelicoflavus ZG0656 (89/92) --/-- CmmSu 353 sulfotransferase Sfla_0165, S. flavogriseus ATCC 33331 (87/92) 87/92 CmmE 263 carboxymethylproline synthase ThnE, S. cattleya NRRL 8057 (69/80) 93/96 CmmF 338 N-acetyltransferase ThnF, S. cattleya NRRL 8057 (58/66) 86/89 CmmG 263 oxidoreductase ThnG, S. cattleya NRRL 8057 (70/80) 87/92 CmmH 229 HAD-superfamily hydrolase ThnH, S. cattleya NRRL 8057 (57/69) 82/87 CmmI 466 LysR family transcriptional regulator ThnI, S. cattleya NRRL 8057 (51/64) 83/87 CmmJ 487 Transporter protein ThnJ, S. cattleya NRRL 8057 (56/68) 89/93 CmmK 680 radical SAM methyltransferase ThnK, S. cattleya NRRL 8057 (79/86) 95/96 CmmL 394 radical SAM methyltransferase ThnL, S. cattleya NRRL 8057 (67/72) 82/86 CmmM 459 Carbapenam synthetase ThnM, S. cattleya NRRL 8057 (64/76) 86/91 CmmN 369 AFD class I superfamily protein, acyl-CoA synthase ThnN, S. cattleya NRRL 8057 (86/91) 95/97 CmmO 467 aldehyde dehydrogenase ThnO, S. cattleya NRRL 8057 (75/82) 90/94 CmmP 482 radical SAM methyltransferase ThnP, S. cattleya NRRL 8057 (83/91) 97/98 CmmQ 259 putative oxigenase ThnQ, S. cattleya NRRL 8057 (72/81) 93/94 Cmm17 292 enoyl-CoA hydratase Kyg_13766, Acidovorax sp. NO-1 (34/46) 89/93 CmmR 228 nudix hydrolase ThnR, S. cattleya NRRL 8057 (55/66) 75/82 CmmT 388 hydrolase ThnT, S. cattleya NRRL 8057 (69/75) 87/90 CmmS 334 β-lactamase Orf11, S. clavuligerus ATCC 27064 (63/71) 80/87 CmmPah 298 proclavaminate amidino hydrolase PAH, S. clavuligerus ATCC 27064 (52/65) 86/90 Cmm22 227 two-component system response regulator SPW_1836, Streptomyces sp. W007 (84/90) --/-- Cmm23 506 two-component system sensor kinase Sacte_5700, Streptomyces sp. SirexAA-E (89/93) --/-- Cmm24 217 unknown Sacte_5699, Streptomyces sp. SirexAA-E (74/79) --/-- Orf25 1094 putative peptidase Sacte_5698, Streptomyces sp. SirexAA-E (81/88) --/-- Orf26 1029 putative glycosyl hydrolase Szn_17732, Streptomyces zinciresistens K42 (78/88) --/-- Orf27 294 putative RNA polymerase sigma factor Sfla_6134, S. flavogriseus ATCC 33331 (88/93) --/-- Orf28 441 putative serine/threonine phosphatase Sfla_6135, S. flavogriseus ATCC 33331 (85/91) --/-- Orf29 164 putative MarR family transcriptional regulator S.fla_6136, S. flavogriseus ATCC 33331 (93/95) --/-- Open in a separate window a --/--: not in the cluster.

Techniques: Sequencing